畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (12): 2733-2744.doi: 10.11843/j.issn.0366-6964.2018.12.023

• 基础兽医 • 上一篇    下一篇

基于网络药理学分析归芪益母汤治疗牛气血两虚证的作用机制

董朕1,2, 白东东3, 刘利利1,2, 白玉彬1,2, 王玮玮1,2, 张继瑜1,2, 周绪正1,2*   

  1. 1. 中国农业科学院 兰州畜牧与兽药研究所农业部兽用药物创制重点实验室, 兰州 730050;
    2. 中国农业科学院 兰州畜牧与兽药研究所甘肃省新兽药工程重点实验室, 兰州 730050;
    3. 中国农业科学院 兰州畜牧与兽药研究所甘肃省中兽药工程技术研究中心, 兰州 730050
  • 收稿日期:2018-05-08 出版日期:2018-12-23 发布日期:2018-12-23
  • 通讯作者: 周绪正,研究员,E-mail:zhxuzheng@163.com
  • 作者简介:董朕(1991-),男,辽宁铁岭人,硕士生,主要从事兽医毒理学与药物安全性评价研究,E-mail:13104100291@163.com
  • 基金资助:

    国家科技支撑计划项目(2015BAD11B01-01);现代农业产业技术体系建设专项(CARS-37)

The Mechanism of Gui Qi Yimu Decoction Powder in Treating Cow Qi and Blood Two Deficiency Syndrome Based on Network Pharmacology

DONG Zhen1,2, BAI Dong-dong3, LIU Li-li1,2, BAI Yu-bin1,2, WANG Wei-wei1,2, ZHANG Ji-yu1,2, ZHOU Xu-zheng1,2*   

  1. 1. Key Laboratory of Animal Drug production in Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences(CAAS), Lanzhou 730050, China;
    2. Key Laboratory of New Veterinary Medicine Engineering in Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, CAAS, Lanzhou 730050, China;
    3. Veterinary Medicine Engineering Technology Research Center Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, CAAS, Lanzhou 730050, China
  • Received:2018-05-08 Online:2018-12-23 Published:2018-12-23

摘要:

为探讨归芪益母汤的疗效及配伍机制,通过中药系统药理学数据库和分析平台(TCMSP)研究了三种中药的化学成分和作用靶点,构建了复合靶网络、蛋白质相互作用(PPI)网络和靶通路网络,探讨了归芪益母汤的作用机制。通过分析,共筛选出24个成分,249个与三种中药有关的靶点。PPI网络包含182个靶点,其中包括TNF、IL8、HSP90AA1、MAPK1、F3、ENSBTAG00000014921、PSMD3、TP53等关键靶点。共有73个基因本体(GO)条目,包括61个生物过程条目、9个分子功能条目和3个细胞成分条目。此外,还有11条KEGG通路,涉及肿瘤中的通路、T细胞受体信号通路、Toll样受体信号通路、细胞凋亡、钙信号通路等。研究结果初步验证了归芪益母汤的基本药理作用及相关机制,为进一步研究其作用机制奠定了基础。

Abstract:

The aim of our study was to explore the curative effect and compatibility mechanism of Gui Qi Yimu decoction. The chemical constituents and action targets of three kinds of traditional Chinese medicines were studied by pharmacological database and analysis platform of TCMSP. The compound target network, protein interaction PPI network and target pathway network were constructed. Through screening, a total of 24 constituents, 249 targets related to three kinds of traditional Chinese medicines were selected. PPI network contains 182 targets, including TNF, IL8, HSP90AA1, MAPK1, F3, ENSBTAG00000014921, PSMD3, TP53 and other key targets. There are 73 gene Noumenon (GO) entries, including 61 biological process items, 9 molecular functional items and 3 cellular component entries. In addition, there are 11 KEGG pathways, including tumor pathway, T cell receptor signal pathway, Toll like receptor signal pathway, apoptosis, calcium signal pathway, and so on. The results of the study preliminarily verified the basic pharmacological effects and related mechanisms of Guiqi Yimu decoction, which laid a foundation for further study of its action mechanism.

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